Direct inoculation of positive blood cultures using the Phoenix system for antimicrobial susceptibility testing of both Gram-positive and Gram-negative bacteria

摘要

Vacutainer tube as described previously and adjusted to the turbidity equivalent of 0.5 McFarland. A total of 25 ul of this suspension was transferred to Phoenix AST broth (BD) supplemented with one drop of Phoenix AST Indicator solution (BD) for staphylococci, enterococci and Gram- negative bacteria or with Phoenix AST-S Indicator solution for streptococci (BD). Next, these suspensions were inoculated into the appropriate BD Phoenix panels. Once completely filled, the panels were logged and loaded into the Phoenix system. We used a BD Expert system, which analysed the antimicrobial susceptibility profiles for unusual results and potentially erroneous results were flagged for verification.