Antioxidant and Protective Effects of Angiparsa?￠, a New Herbal Drug, on Hydrogen Peroxide-Induced Oxidative Stress in HepG2 and A549 Cell Lines

摘要

In the present study, free-radical-scavenging and antioxidant activities of Angiparsa?￠, an herbal extract which has been registered as a therapeutic drug for diabetic foot ulcer, were examined. We investigated the protective effect of Angiparsa?￠ on cytotoxicity produced by exposure to H2O2 (300 ?μM) in HepG2 and A549 cell lines, using MTT assay, glutathione (GSH) content analysis, and lipid peroxidation evaluation. Angiparsa?￠ concentrations of 1 - 20 ?μg/mL effectively scavenged DPPH radicals in a dose-dependent manner, while hydroxyl-radical-scavenging activity was only observed at high doses. Following a 4-hour cell treatment with H2O2, a noticeable decline in cell viability was observed, which was alleviated by Angiparsa?￠ (pre-incubated for 24 hours) at high doses. Angiparsa?￠ significantly reduced GSH content and products of lipid peroxidation, resulting from H2O2 exposure. These findings suggest that Angiparsa?￠ exhibits positive antioxidant activity in vitro and can attenuate H2O2-induced cytotoxicity; in fact, this may represent the cellular mechanisms for its cytoprotective activity.