Identification of Toxoplasma gondii Excretory-Secretory Antigens from Propagation in Mice as Toxoplasmosis Vaccine Candidate

摘要

The aim of this research is to get some ESAs of T. gondii tachyzoite from propagation in mice and ESAs having the character of immunogenic as candidate of toxoplasmosis vaccine. The long-range target of this research is get subunit vaccine of toxoplasmosis. The methods used cover: propagation of T. gondii in mice and cropping ESAs at intraperitoneal fluid and fractination ESAs by SDS-PAGE, antibody making to ESAs, and determination antigenicity by western blot. The results of research founded 13 ESAs: 125.5; 100.9; 70.2; 56.7; 45.6; 40.4; 35.3; 34.1; 29.5; 27.4; 24.9; 20.7 and 19.7 kDa. ESAs having the character of antigenic were molecule weighing 100.9; 56.7; 35.3; 34.1; 24.9; 20.7 and 19.7 kDa, and 100.9; 56.7; 24.9; 20.7 and 19.7 kDa represent major antigenic