Regeneration via Direct Organogenesis from Leaf Segments of Eggplant ( Solanum melongena L.)

摘要

Direct organogenesis from leaf explant of Indian variety of Solanum melongena L. (PLR1) was successfully achieved. Eggplant leaves cultured for 10?12 days on MS medium supplemented with (2iP) 2.0 mg L^?1 and Naphthalene acetic acid (NAA) 1.0 mg L^?1 induced high frequency shoot organogenesis (79-81%) and favored shoot elongation. Shoots developed from leaf explant directly after two weeks of explant inoculation. Shoot proliferation reached maximum in the presence of 2.0 mg L^?1 2iP with induction of 4.5-5.0 average mean number of shoots per explant. High frequency of root induction (83-85%) was observed on medium supplemented with 1.0 mg L^?1 Naphthalene acetic acid (NAA) with 6-7 mean number of roots whereas increase in the level of (IBA) Indole butyric acid (2.0 mg L^?1) resulted in induction of roots along with development of callus from the base of the shoots. The plantlets with developed roots are hardened with 2:1 ratio of soil and vermiculite shifted to controlled environmental growth chamber with 8 h photoperiod for acclimatization and after ten days the plantlets are transferred to field condition. This protocol can be efficiently used for mass multiplication and also for regeneration of genetically transformed Solanum melongena tissue.