CdS nanocrystals as fluorescent probe for detection of dolasetron mesylate in aqueous solution: Application to biomedical analysis

摘要

A simple and straightforward method for the determination of dolasetron mesylate (DM) in aqueous solution was developed based on the fluorescence quenching of 3-Mercaptopropionic acid (MPA) capped CdS quantum dots (QDs). The structure, morphology, and optical properties of synthesized QDs were characterized by using UV-Vis absorption spectroscopy, fluorescence spectroscopy, transmission electron microscopy (TEM) and dynamic light scattering (DLS) measurements. Under the optimum conditions, the MPA-CdS QDs fluorescence probe offered good sensitivity and selectivity for detecting DM. The probe provided a highly specific selectivity and a linear detection of DM in the range of 2–40 μg/mL with detection limit (LOD) 1.512 μg/mL. The common excipients did not interfere in the proposed method. The fluorescence quenching mechanism of CdS QDs is also discussed. The developed sensor was applied to the quantification of DM in urine and human serum sample with satisfactory results. Graphical abstract Highlights ? MPA capped CdS QDs as a selective fluorescence probe for dolasetron mesylate (DM) in aqueous solution. ? Fluorescence quenching of CdS QDs by DM is due to nonradiative recombination of electron and hole. ? Very low detection limit (LOD=1.512 μg/mL). ? The probe can be used to determine DM in urine and human serum sample.