CdTe量子点荧光探针在生物分析中的应用

摘要

目的 探讨巯基丙酸包被的CdTe量子点在生物分析中应用价值.方法 水相中合成CdTe量子点并进行巯基丙酸包被,并对其进行了荧光发射光谱及透射电子显微镜成像表征；将量子点与亲和素连接、制备成量子点荧光探针；应用激光扫描共聚焦显微术观察量子点荧光探针标记小鼠腹腔巨噬细胞(peritoneal macrophage,PMΦ)MHCⅡ抗原的表达；以H22肝癌细胞为靶细胞,MTT法观察量子点的生物相容性.结果 CdTe量子点粒径均匀,具备良好的光学性能；量子点荧光探针标记细胞具有较强的荧光表达；量子点在一定浓度范围内对细胞的毒性小.结论量子点荧光探针能对固定的组织细胞进行标记,同时具有较好的生物相容性,可对活细胞进行直接或动态标记.%Objective To investigate the application of CdTe quantum dots as fluoresent probes in Biological analysis. Methods Prepared CdTe quantum dots in aqueous phase and coating with MPA. Exosyndrome QDs with TEM and Fluorspectro-photometer. Binded QDs to avidin, purified the connective and prepared it as flurescent probe. LSCM was used to observe the QDs fluorescent probe label expression of MHCⅡ antigen on PMΦ and used MTT to observe the biocompatibility of CdTe quantum dots coated with MPA using the H22 cells as target cells. Results The particle diameter of CdTe quantum dots prepared in aqueous phase was well-distributed and it had good photology performance. QDs had little cytotoxicity within some concentration range. Conclusion CdTe QDs has good biocompatibility. It's not only fit for immunofluorescence label of fixed histiocyte but also can satisfy the experiment desire of direct or dynamic label of live cells.