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Effects of Boron and Cold Stress on Germination of Almond Pollen in Vitro Culture

机译:硼和冷胁迫对体外培养杏仁花粉萌发的影响

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This study was carried out in order to determine the effects of boron and cold stress on pollen germination of almond hybrids in vitro culture, two different media including the absence and presence of boron (0 mg/l, and 100 mg/l) in 10% sucrose and 1% agar medium at 24°C in dark conditions. The results showed that different treatments had a significant effect on the germination percentage. The highest pollen germination percentage in H24 and S8 almond hybrids was recorded as 98.35 % and 97%, 34% in vitro culture medium containing 100 ppm boric acid, 10% sucrose +1% agar, whereas these amounts were 87.56% and 89.46% in the basic medium without the presence of boric acid, respectively. In conclusion, in vitro pollen germination of almond was inhibited in the medium culture without boron and in cold stress, while it was increased in the medium with boron. Thus, boron can increase the almond yield.
机译:进行这项研究是为了确定硼和冷胁迫对体外培养杏仁杂种花粉萌发的影响,两种不同的培养基包括在10中不存在和存在硼(0 mg / l和100 mg / l)的情况。在黑暗条件下于24°C的蔗糖浓度和1%琼脂培养基浓度。结果表明,不同处理对发芽率有显着影响。在H24和S8杏仁杂种中最高的花粉发芽率记录为98.35%和97%,34%的体外培养基中含有100 ppm的硼酸,10%的蔗糖+ 1%琼脂,而这些含量分别为87.56%和89.46%。不含硼酸的基本培养基。总之,在无硼和冷胁迫的培养基中,杏仁的体外花粉萌发受到抑制,而在含硼的培养基中,杏仁的花粉萌发受到抑制。因此,硼可以增加杏仁的产量。

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