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Optimization of ultrasonic extraction by response surface methodology combined with ultrafast liquid chromatography–ultraviolet method for determination of four iridoids in Gentiana rigescens

机译:响应面法结合超快速液相色谱-紫外法优化超声波法测定香根草中四种鸢尾酮的含量

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Abstract Gentiana rigescens is a rich source of iridoids and is commonly used as a folk medicine for treatment of hepatitis and cholecystitis for over 1000 years. A rapid ultrafast liquid chromatography–ultraviolet method was developed for simultaneous determination of four major iridoid glycosides in G. rigescens. Response surface methodology based on the Box–Behnken design was applied to optimize the extraction conditions of iridoid glycosides. Using the Shim-Pack XR-ODS III, four iridoid glycosides were efficiently separated with an acetonitrile:0.1% formic acid aqueous solution gradient at a flow rate of 0.25?mL/min for 8 minutes. All the regression equations revealed a good linear relationship (R2??0.9995). The intraday and interday variations were 1.95%. The recoveries ranged from 99.7% to 103.2%. The optimal extraction conditions were as follows: methanol concentration, 82%; the ratio of liquid to solid material, 68:1 (mL/g); and extraction time, 32 minutes. The yield of the four iridoid glycosides under the optimal process was found to be 63.08?mg/g, which was consistent with the predicted yield. In addition, the total content of 50 cultivated samples from Lincang, Yunnan, China, was within the range of 33.6–113.26?mg/g, which provides a more reasonable foundation for utilization of G. rigescens.
机译:摘要秦G(Gentiana rigescens)是一种丰富的鸢尾素来源,在过去的1000多年中一直被用作民间药物,用于治疗肝炎和胆囊炎。建立了一种快速超快速液相色谱-紫外法,用于同时测定rigescenss中四种主要的环烯醚酮苷。基于Box–Behnken设计的响应面方法被用于优化虹彩苷的提取条件。使用Shim-Pack XR-ODS III,用乙腈:0.1%甲酸水溶液梯度以0.25?mL / min的流速有效分离8个环烯醚苷。所有回归方程均显示出良好的线性关系(R2≥0.9995)。盘中和盘中差异<1.95%。回收率从99.7%到103.2%。最佳提取条件如下:甲醇浓度为82%;甲醇为80%。液体与固体物质之比为68:1(mL / g);提取时间为32分钟。在最佳工艺下,四种鸢尾苷的收率为63.08?mg / g,与预测的收率一致。此外,来自中国云南临沧的50个栽培样品的总含量在33.6–113.26?mg / g的范围内,这为rigescenss的利用提供了更合理的基础。

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