High Throughput DNA-DNA Microarray Chip Strategy for Detection and Identification of Enteropathogenic bacteria

摘要

The usability of the DNA microarray system for the specific detection of bacteria based on their unique genes was systematically evaluated with a model system composed of two pathogenic strains and two species specific oligonucleotide probes. Escherichia coli O157: H7 and Salmonella enterica are pathogens which have very low infectious doses (as low as 10 cells), and these bacteria often exist within complex biological matrixes. Detection and identification of these pathogenic bacteria in less number was achieved. Bacteria was subjected to whole genome multiplication and labeled while amplifying the specific partial target gene sequence itself. Microarry chips were printed by free hand method and used for hybridization. This culture independent detection method could be fastening the diagnosis term for the swift food material quality control and therapeutic purpose too.