Optimization of cellulase enzyme production from corn cobs using Alternaria alternata by solid state fermentation

摘要

Cellulase is an important ind ustrial enzyme which can be obtained from cheap agrowastes. Pakistan is an agriculture country, producing tons of waste in the form o f wheat straw, rice bran, sugarcane bagasee, corn cobs, corn stover etc. The aim of the present study was to produce cellulase by using abundant agrowastes like corn cobs. The conditions were optimized by using corn cobs and culturing Alternaria alternata with solid state fermentation. Different incubation times (1-7days), temperatures (25 0 C, 30 0 C, 35 0 C and 40 0 C) and pHs (3.0-9.0) were experimented for the production of cellulase. The optimum culture conditions were 96 hrs of incubation at 35 0 C and pH 6.0, giving enzyme activities of 15.06 μ g/ml, 31.2406 μ g/ml, 26.4106 μ g/ml, respectively.