首页> 外文期刊>Journal of Applied Glycoscience >Screening Method for Novel Rice Starch Mutant Lines Prepared by Introducing Gene Encoding Starch Synthase IIa and Granule-bound Starch Synthase I from Indica Cultivar into a Branching Enzyme IIb-Deficient Mutant Line
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Screening Method for Novel Rice Starch Mutant Lines Prepared by Introducing Gene Encoding Starch Synthase IIa and Granule-bound Starch Synthase I from Indica Cultivar into a Branching Enzyme IIb-Deficient Mutant Line

机译:将coding稻品种编码淀粉合酶Ⅱa和颗粒结合淀粉合酶Ⅰ的基因导入分支酶Ⅱb缺陷型突变株中筛选新型水稻淀粉突变株

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The structure and properties of starch reserves in rice seeds are strongly affected by deficiencies in specific starch biosynthetic enzymes, which are highly expressed in storage tissues. Rice lines with unique seed starches should be utilized for food and industrial applications in the near future. We are currently developing novel rice mutant lines with distinct starch properties by introducing specific genes from different cultivars into mutant lines and by producing multiple combinations of single mutant lines. Obtaining the homozygous genotypes of the target genes is necessary during the screening process of these materials. In this study, we developed an effective, accurate screening method for identifying rice lines with novel starch composition. Specifically, we produced a novel mutant line in which we introduced genes encoding starch synthase IIa (SSIIa) and granule-bound starch synthase I (GBSSI) from indica cultivar into a starch branching enzyme IIb (BEIIb)-deficient mutant line.
机译:水稻种子中淀粉储备的结构和性质受到特定淀粉生物合成酶缺乏的强烈影响,这些淀粉在储存组织中高度表达。不久的将来,具有独特种子淀粉的水稻系应用于食品和工业应用。我们目前正在通过将来自不同品种的特定基因引入突变株系并通过产生单个突变株系的多种组合来开发具有独特淀粉特性的新型水稻突变株系。在这些材料的筛选过程中,必须获得目标基因的纯合基因型。在这项研究中,我们开发了一种有效,准确的筛选方法,以鉴定具有新型淀粉成分的水稻系。具体来说,我们产生了一个新的突变株系,其中我们将来自in品种的编码淀粉合酶IIa(SSIIa)和颗粒结合淀粉合酶I(GBSSI)的基因引入了淀粉分支酶IIb(BEIIb)缺失的突变株系中。

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