Effect of 4-Nitrophenol on Male Mice Reproductive Function

摘要

4-Nitrophenol (PNP) has deleterious effects on mammalian physiological functions. The objective of the study is to identify the effect of 4-Nitrophenol on male mice reproductive function. About 50 adult Kunming male mice (25-30 g) were randomly divided into 5 groups, administrated (0.1, 10 and 100 mg kgwt-1) orally for 35 days. Semen quality, histological structure of testis and ultrastructure of primary spermatocytes were detected to analysis effect of PNP on the spermatogenesis function. Furthermore, the functions of liver and kidney were determined by testing the blood of biological indicators. The results showed that 0.1 mg kgwt-1 PNP decreased slightly in body weight (p>0.05) but significantly reduced and rogen hormone-sensitive organ weights (p<0.05). However, semen quality (total sperm count, survival, linear motion and acrosome integrity rate), liver function (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total protein and plasma albumin (p>0.05) and renal function (urea, uric acid and creatinine) had no significant changes (p>0.05); 10 and 100 mg kgwt-1 PNP reduced not only significantly body weight, androgen hormone-sensitive organ weights (p<0.05) but also significantly reduced semen quality, liver and renal function and the effect of 100 mg kgwt-1 PNP was stronger than that of 10 mg kgwt -1 PNP (p<0.05); three doses of PNP on the testicular structure and ultra structure of primary spermatocytes had injury effect. With increasing concentrations of PNP, the damage was enhanced. About 100 mg kgwt-1 PNP significantly disrupted testicular structure and ultra structure of the primary spermatocytes. This shows that PNP affects the liver and kidney detoxification function which reduced the detoxification ability of body and damages the process of spermatogenesis.