室内装修挥发性污染物对雄性小鼠生殖功能和精子凋亡的影响

摘要

目的:模拟室内装修主要挥发性物质的污染环境,探讨室内装修主要挥发性污染物甲醛、苯系物及氨对雄性小鼠生殖功能的影响.方法:选取雄性 ICR小鼠20只,随机平均分为实验组和对照组,实验组小鼠进行甲醛(60 mg·m-3)、二甲苯(50 mg·m-3)和氨(40 mg·m-3)经呼吸道混配静式染毒,同时对照组小鼠放入空气填充的静式染毒柜中.每天固定时间称量小鼠体质量并记录,连续染毒35 d.染毒期间每天观察小鼠行为学变化.收集小鼠附睾尾部精子并计数,检测精子密度及活力;采用 Diff-Quick染色法染色,计算精子畸形率;采用流式细胞术(FCM)检测小鼠附睾尾部精子凋亡率;采用 Western blotting法检测小鼠睾丸组织中细胞凋亡相关因子Caspase-9和Cleaved Caspase-3蛋白表达水平.结果:染毒28 d后,实验组小鼠体质量与对照组比较明显降低(P<0.01);染毒35 d后,实验组小鼠精子浓度、活力及精子畸形率与对照组比较均明显下降(P<0.01);与对照组比较,实验组小鼠精子凋亡率明显升高(P<0.01).实验组与对照组小鼠睾丸组织中Caspase-9和Cleaved Caspase-3蛋白表达水平比较差异无统计学意义(P>0.05).结论:室内装修主要污染物能够明显影响雄鼠附睾中精子质量.%Objective:To simulate the pollution environment of indoor decoration volatile pollutants,and to explore the influence of three main indoor decoration volatile pollutants formaldehyde,xylene and ammonia in the male mouse reproductive function.Methods:A total of 20 male ICR mice were randomly divided into experimental group(exposed to 60 mg·m-3formaldehyde,50 mg·m-3xylene and 40 mg·m-3ammonia mixed in the static type canister)and control group(put into the air-filled static type canister)(n=10).Each mouse was weighed at set times every day,exposed for 35 d continuously.The behavior changes of the mice were also observed everyday. The epididymal cauda sperms were collected to detect the sperm density and vitality.The sperm malformation rate was detected by Diff-Quick dyeing method.The apoptotic rate of epididymal cauda sperms was detected by flow cytometry(FCM).Then Western blotting method was used to determine the expression levels of Caspase-9 and Cleaved Caspase-3 proteins in testis tissue of the mice.Results:Four weeks after the exposure to pollutants,the body weight of the mice in experimental group was significantly lower than that in control group(P<0.01).The concentration and vitality of sperm malformation rate of the mice in experimental group were significantly lower than those in control group(P<0.01)35 d after exposure.Compared with control group,the apoptotic rate of sperm of the mice in experimental group was significantly increased(P< 0.01).However,the expression levels of Caspase-9 and Cleaved Caspase-3 in the testis tissue of the mice in experimental group and control group had no significant differences(P>0.05).Conclusion:The main pollutants in indoor decoration can significantly influence the quality of spermatozoa in the epididymis.