Inhibition by Chlorpromazine, Metals and I-Ascorbic Acid of Calcium-ATPase and Magnesium-ATPase in Bovine Adrenal Medullary Microsomes

摘要

References(33) Cited-By(5) Effects of chlorpromazine, metals and I-ascorbic acid (AA) on Ca2+-ATPase and Mg2+-ATPase in microsomal and granular fractions obtained from the bovine adrenal medulla were studied. Marker enzyme analysis on microsomal subfractions in a discontinuous sucrose density gradient showed a correlation of distribution between ATPase activities and plasma membrane. The two ATPase activities in such plasma membrane-rich microsomes were reduced by chlorpromazine, Hg2+ and Cu2+ (0.3 mM of each), and their effects were greater on the Mg2+-ATPase activity. Zn2+(0.3 mM) also reduced only the Mg2+-ATPase activity. AA (3 mM) reduced the two ATPase activities to an equal extent. Nevertheless, the inhibitions of ATPases by Hg2+, Cu2+ and Zn2+ were decreased, unaltered and additively enhanced in combination with AA, respectively. We also observed high Mg2+-ATPase activity in the granule-rich fraction, but this ATPase activity was unaffected by all of the above agents. These results indicate that Mg2+-ATPase in the plasma membrane-rich microsome of adrenal medulla is inhibited by chlorpromazine, Hg2+, Cu2+ and Zn2+ more significantly than Ca2+-ATPase, but Mg2+-ATPase in the granular fraction is unaffected, and that AA changes the potency of inhibition by some metals of ATPases diversely.