Estimation of phenolic contents and anti-oxidant activity of Begonia trichocarpa

摘要

This work was designed to give a taxonomical description, estimation of anthocyanin content, phenol content and antioxidant activity of Begonia trichocarpa dazll belong to Begoniaceae. The taxonomical description shows leaves are 8-10 x 3-5 cm, ovate-oblong, acuminate, obliquely cordate at base, hirtus, petiole 3-4 cm long, reddish, succulent, male and female flower with perianth segments, Ovary Subglobose, to 3.5 mm long, with semi-obovate wings on either sides. The leaf and stem contain 23.71% and44.08% of anthocyanin content respectively. The phenolic content of methanol extract was found to be high with 46.96% followed by chloroform 17.5%, ethyl acetate 12.89% and petroleum ether 8.12%. A dose dependent anti oxidant activity was found in both DPPH free radical scavenging method and Nitric acid scavenging method. DPPH assay of methanol extract and ethyl acetate extract shows maximum % inhibition 53.0% and 50.93% at the concentration of 400μg/ml respectively, where as ascorbic acid exhibit 70.36% and Ic50 values were 335.23μg/ml, 370.74μg/ml and 16.8407 μg/ml respectively. Nitric acid scavenging activity of methanol extract and ethyl acetate extract shows maximum % inhibition 46.53 % 27.36 % and ascorbic acid exhibit 53.34% ,Ic25 values were found 150.87 μg/ml,509.16 μg/ml and ascorbic acid 0.633 μg/ml.