Identification and determination of different alkaloids from Atropa belladonna L. by Gas chromatography method

摘要

Background & Aim A. belladonna (family Solanaceae) is one of important pharmaceutical plants which contain tropane a??alkaloids. Tropane alkaloids are distinct group of secondary metabolites of the a??Solanaceae family. ??The most important alkaloids of A. belladonna are atropine and hyoscine that are used extensively because of their medicinal properties. Therefore Identification and a??Determinationa?? a??of Different Alkaloids rate by Gas chromatography (GC) Method in Iran various areas are necessary. Experimental The accessions were collected from five different regions including Ardabil, Marzanabad, Tonekabon, Karaj and Ramsar (Iran) and all of plant organs were dried. Using a??change of pHa?? a??and solvents, the alkaloids contents were extracted and the identified and finally a??the amount of each alkaloid was determined. Results & Discussion Atropine contents in the accessions of Ardebil, Marzanabad, Tonekabon, Karaj, and Ramsar were a??determined 1.8, 1.62, 1.01, 0.76 and 0.67 % respectively. Hyoscine contents were determined 0.47, a??a??0.23, 1.23, 0.16 and 0.09 % respectively. And amount of both total alkaloids were determined 2.66, a??a??1.85, 2.24, 0.92 and 0.76 % respectively. In this study, the highest amount of atropine (1.8 %), and both a??total alkaloids which extracted from all dried plant organs belong to the accessions from Ardebil and the a??highest amount of hyoscine (0.46 %) were determined in the accessions from Tonekabon. The lowest a??amount of hyoscine, atropine and both total alkaloids belong to the accessions were from Ramsara??.???? Industrial and practical recommendations According to the findings of this study, for extraction more atropine and collection of atropine and ????hyoscine from Atropa bladonna, the samples of Ardabil region can be recommended.