Effect of exogenous progesterone treatment on ovarian steroid hormones and oxidant and antioxidant biomarkers during peak and low breeding seasons in dromedary she-camel

摘要

Background: Research about the effects of progesterone (Psub4/sub) and the relationship of Psub4/sub to oxidative stress has been achieved in ruminants but not enough in camels Aim: This study evaluated the effect of exogenous Psub4/sub hormone using CIDR for 7 days on blood concentrations of steroid hormones and oxidative status of dromedary she-camels during peak and low breeding seasons. Materials and Methods: The present work was conducted on ten dark dromedary she-camels which were synchronized using a controlled internal drug release (CIDR) for 7 days as a reproductive management tool during peak breeding (November-April) and low breeding season (May-October). The blood samples were collected each other day from CIDR insertion until the end of experiment 5 days after the removal of CIDR. Camels were examined for Psub4/sub, estradiol (Esub2/sub), and testosterone (T) as well as malondialdehyde (MDA) as indicator of lipid peroxidation and nitric oxide, superoxide dismutase (SOD), and glutathione-S-transferase as antioxidant markers. Results: Results revealed that Psub4/sub was higher during peak breeding season than low breeding season. While the levels of Psub4/sub increased during CIDR insertion and declined at CIDR removal and thereafter during breeding season, its concentrations declined after CIDR application during the non-breeding season. On the other hand, blood Esub2/sub and testosterone levels decreased after CIDR insertion in both high and low breeding seasons with higher serum Esub2/sub concentrations during the peak than the low breeding season. MDA concentrations and SOD activities were significantly (p4/sub treatment through CIDR in dromedary camels could be more efficient during breeding season than non-breeding season, and effects on circulating oxidant/antioxidant biomarkers and their return to normal levels might refer to the adaptation of camels to CIDR by modulating their oxidant and antioxidant levels.