Cloning and Expression Patterns of MRFs and Effect of Replacing Dietary Fish Oil with Vegetable Oils on MRFs Expression in Grass Carp (Ctenopharyngodon idellus)

摘要

In order to investigate characterization and tissue distribution of myogenic regulatory factors (MRFs) in grass carp (Ctenopharyngodon idellus), full-length of Myf5, MyoG, Myf6 cDNAs were cloned and expression patterns of MRFs in different tissues were studied. Meanwhile the effect of dietary oil sources (fish oil,FO；linseed oil, LO; groundnut oil, GO; olive oil, OO) on MRFs gene expression in muscle of grass carp were studied by feeding experiment. The results showed that nucleotide sequence of Myf5, MyoG and Myf6 contained a basic DNA-binding motif and a helix-loop-helix dimerization domain (bHLH) respectively. MRFs were mainly expressed in muscle and a little were expressed in other tissues tested. In red muscle MRFs was mostly expressed in OO group and in white muscle they were mostly expressed in GO group. These results showed that MRFs gene and amino acid in grass carp shared high conservation and it mostly expressed in muscle. Dietary oil sources have a great impact on expression of MRFs genes in muscle of grass carp, probably indicating that OO or GO would be more suitable than FO to be oil sources in diet of grass carp.