Laboratory Use of Lectin Mitogens for Mitotic Stimulation of Human Lymphocytes

摘要

Lectins are believed to act as modulations of cell substratum interactions and to be essential for the normal differentiation and growth of all multicellular humans and animals. Although several lectins have been reported from microfungi, many more genera remain unexplored and their physiological role is also uncertain. The aim of this laboratory work was to make a comparison between self-made lectins (Indigenous) and commercial ones, following High Resolution Cell Synchronization technique (HRCS). Cytogenetic studies were performed in 175 normal healthy blood donor individuals of both genders and statistical analysis was performed. Our results indicated that the preparation of fresh phytohemagglutinin at the time of cell division and cell culture procedure reveals a satisfactory score. The overall frequency of mitotic index in our study was higher when compared with commercial imported Lectins (p 0.05). The significant differences in the results may be due to fresh preparation. However, the cost effective, easy and nearest approach of this indigenous product, as well as the high demand for this product, among health care services can be considered. Highlights: Lectins act as essential factor of the normal differentiation and growth of all humans and animals. Phytohemagglutinin (PHA) is a lectin (mucoprotein) from Phaseolus vulgaris . Crude extract of PHA could be used in human leucocyte cultures as mitotic stimulator. The indigenous PHA have used to identify chromosome preparation in normal conditions and malignancies.