Enhanced Production of Palmarumycins C12 and C13 in Mycelial Liquid Culture of the Endophytic Fungus Berkleasmium sp. Dzf12 with In situ Macroporous Resin Adsorption

摘要

Purpose: To evaluate in situ macroporous resin adsorption for enhancement of palmarumycins C₁₂ and C₁₃ production in mycelial liquid culture of the endophytic fungus Berkleasmium sp. Dzf12. Methods: Ten macroporous adsorption resins (D-101, D1300, HPD-100, X-5, AB-8, DM130, ADS-17, DA-201, NKA-9 and S-8) were tested for absorption of palmarumycins C₁₂ and C₁₃ in mycelial liquid culture of the endophytic fungus Berkleasmium sp. Dzf12. Results: Among the resins, DA-201 showed the most significant enhancing effect on accumulation of palmarumycins C₁₂ and C₁₃ in mycelial liquid culture of endophytic fungus Berkleasmium sp. Dzf12. When resin DA-201 was applied to the medium at a concentration of 4.17 % on day 11 and then harvested on day 15, the maximal yield of palmarumycins C₁₂ and C₁₃ was 149.86 and 55.78 mg/L, which correspond to 70.69- and 1.82-fold higher than for control (2.12 and 30.70 mg/L, respectively). Approximately 95.81 % of palmarumycin C₁₂ and 87.20 % of palmarumycin C₁₃ were distributed in resin DA-201. Conclusion: The results indicate that in situ resin adsorption is an effective strategy for enhancing the production of palmarumycins C₁₂ and C₁₃, and also for facilitating their recovery from the mycelial liquid culture of Berkleasmium sp. Dzf12.