Enhancement of diepoxin ζ production with in situ resin adsorption in mycelial liquid culture of the endophytic fungus Berkleasmium sp. Dzf12 from Dioscorea zingiberensis

摘要

Berkleasmium sp. Dzf12, an endophytic fungus from Dioscorea zingiberensis, is a high producer of the spirobisnaphthalene diepoxin ζ. However, diepoxin ζ produced by Berkleasmium sp. Dzf12 was retained as both the intracellular and extracellular product. This study was to evaluate an in situ resin adsorption for enhancement of diepoxin ζ production in mycelial liquid culture of Berkleasmium sp. Dzf12. Diepoxin ζ production was most effectively enhanced by macroporous resin AB-8 among five test resins. The highest diepoxin ζ yield reached 448.6 mg l−1 that was 1.4 fold of the control (329.7 mg l−1), when 1.5 g of resin AB-8 was added to 30 ml medium in each flask on day 11 of culture and in a period of 40 h for adsorption. The results show that in situ resin adsorption is an effective strategy for enhancing diepoxin ζ production and also facilitating its recovery in mycelial liquid culture of Berkleasmium sp. Dzf12.