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首页> 外文期刊>The Journal of Veterinary Medical Science >Composition of fecal microbiota of laboratory mice derived from Japanese commercial breeders using 16S rRNA gene clone libraries
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Composition of fecal microbiota of laboratory mice derived from Japanese commercial breeders using 16S rRNA gene clone libraries

机译:使用16S rRNA基因克隆文库衍生自日本商业饲养者的实验小鼠粪便微生物群的组成

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References(25) The fecal microbiota of six mice derived from three Japanese commercial breeders was analyzed by using 16S rRNA gene clone libraries to construct a database for analyzing the gut microbiota of laboratory mice. The 566 clones were obtained from the clone libraries generated from the fecal DNA samples derived from BALB/c, C57BL/6N, DBA/2 and ICR mice. Among these 566 clones, there were 446 unique 16S rRNA gene sequences. When grouped at the 98% similarity level, the 446 unique sequences consisted of 103 Clostridiales, 43 Bacteroidales, 5 Lactobacillus and 3 Erysipelotricaceae, as well as sequences from 11 other phyla.
机译:参考文献(25)通过使用16S rRNA基因克隆文库构建了用于分析实验室小鼠肠道菌群的数据库,分析了来自三个日本商业育种者的六只小鼠的粪便菌群。从从BALB / c,C57BL / 6N,DBA / 2和ICR小鼠衍生的粪便DNA样品产生的克隆文库获得566个克隆。在这566个克隆中,有446个独特的16S rRNA基因序列。当以98%的相似度分组时,这446个独特序列由103个梭菌,43个细菌,5个乳杆菌和3个杜鹃花科以及其他11个门的序列组成。

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