Association of CCND1 Gene c.870G&A Polymorphism with Breast Cancer Risk: A Case-ControlStudy and a Meta-Analysis

摘要

id="Par1" class="Para">Cyclin D1 (CCND1) plays an essential role in regulating the progress of the cell cycle from G1 to S phase. There is a common c.870GA polymorphism in the CCND1 gene. The aim of this study was to investigate the association of CCND1 gene c.870GA polymorphism with breast cancer risk in a case-control study, which followed by a meta-analysis and an in silico analysis. Three hundred and thirty-five subjects composed of 174 women with breast cancer and 161 healthy controls were included in the case-control study. CCND1 gene c.870GA genotyping was performed by PCR-RFLP. Meta-analysis was done for 14 studies composed of 7281 cases and 6820 controls. Some bioinformatics tools were applied to investigate the effects of c.870GA on the mRNA splicing and structure. Our data obtained from case-control study revealed that GA genotype (OR: 1.89, 95%CI: 1.12-3.17, p??=??0.017), AA genotype (OR: 1.95, 95%CI: 1.08-3.53, p??=??0.027), and A allele (OR: 1.44, 95%CI: 1.06-1.95, p??=??0.019) were significantly associated with breast cancer risk. The results of meta-analysis showed a significant association between CCND1 c.870GA polymorphism and breast cancer risk, especially in Caucasian population. In silico analysis revealed that c.870GA transition affect CCND1 mRNA splicing and secondary structure.