Isolation, Characterization and Identification of Microorganisms from Spoilt Carrots Obtained from Ose Market Onitsha, Nigeria

摘要

Studies on the microorganisms associated with spoilt carrots obtained from Ose Market, Onitsha, Nigeria were carried out using standard cultural techniques. Nutrient agar, sabouraud dextrose agar and Eosin methylene blue agar were the growth media for the isolation of the heterotrophic bacteria, fungi and coliforms. The bacteria were identified as Serratia marcescens, Escherichia coli and Corynebacterium bovis while the fungi was identified on the basis of their colonial and microscopic characteristics as Penicillium digitatum, Rhizopus stolonifer, Aspergillus niger and Alternaria alternata. Escherichia coli was predominantly isolated among the bacterial isolates (50%) while Aspergillus niger occurred most frequently than the other fungal species (40%). These organisms may have been introduced to the carrots during growth, harvesting, handling, storage and distribution. The presence of the organisms is a public health risk because of the diseases known to be caused by them. It is therefore imperative that adequate hygienic practices must be put in place during the storage and handling of carrots. Spoilt carrots must also not be consumed as they contain a teaming population of bacteria and fungi, some of which are pathogenic to humans.