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Studies Regarding the Morphological Identification of Fusarium sambucinum Fuckel Isolated from Potato Tubers

机译:马铃薯块茎中镰刀镰刀菌形态鉴定的研究

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Fusarium sambucinum Fuckel - teleomorph Gibberella pulicaris (Fr.) Sacc. - is a common pathogen causing dry rot of stored tubers, in temperate areas. Fusarium dry rot is one of the most important diseases of potato ( Solanum tuberosum L.), affecting the tubers in storage and the seed pieces after planting. To establish strategies for the control of this disease it should be made primarily a correct diagnosis and identification of the pathogen. The purpose of this study is to identify based on morphological characters the Fusarium sambucinum Fuckel isolated from potato tubers. The characters followed are: the colony growth diameters, the culture pigmentation, the shape and the size of the macroconidia, the presence and the absence of the microconidia and of the chlamydospores. The isolates were obtained in two ways: directly from the tuber tissue and from the diseased sprout formed after the tuber was maintain in the damp chamber. In this study, the length and the width of 100 conidia from each Fusarium sambucinum Fuckel isolate were measured, having three isolates obtained directly from the tuber tissue and three isolates from spores formed on the surface of the tuber after keeping them in damp chamber conditions.
机译:Fusarium sambucinum Fuc​​kel-变态赤霉菌(Fr.)Sacc。 -是在温带地区导致储存的块茎干腐的常见病原体。镰刀菌干腐病是马铃薯(Solanum tuberosum L.)的最重要病害之一,影响马铃薯块茎的贮藏和播种后的种子。为了建立控制这种疾病的策略,首先应该对病原体进行正确的诊断和鉴定。这项研究的目的是基于形态特征,鉴定自马铃薯块茎分离的镰刀镰刀菌(Fusarium sambucinum Fuc​​kel)。随后的特征是:菌落的生长直径,培养色素沉着,大分生孢子的形状和大小,是否存在微分生孢子和衣原体孢子。分离物可以通过两种方式获得:直接从块茎组织中获得,以及从将块茎保持在潮湿室内后形成的病芽。在这项研究中,测量了每个镰刀菌Fuckel分离株的100个分生孢子的长度和宽度,其中有3个直接从块茎组织中分离得到的分离物,还有3个分离自块茎表面上的孢子的分离株,将它们置于潮湿的室内条件下。

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