Immunohistochemical expression of anti-CD68 antibody in atherosclerotic plaque

摘要

Inflammation of the vascular wall is an essential event in the development of atherosclerosis, the main leukocytes of the inflammatory infiltrate being the monocyte/macrophages. These cells are very heterogeneous and rapidly change their function in response to signals received from the local environment. Purpose: The aim of this research was to study the immunohistochemical expression of the anti-CD68 antibody at the level of the atherosclerotic plaque. Materials and Methods: We used tissue samples obtained by endarterectomies in patients with symptomatic atherosclerotic lesions. Inflammatory reaction was highlighted in the arterial wall by immunohistochemistry using an anti-CD68 monoclonal antibody that marks normal or pathological monocytes/macrophages. Results and Discussion: The types of analyzed atherosclerotic lesions showed a positive reaction with the anti-CD68 antibody, varying in distribution and intensity in the vascular intima. The CD68 was positive in macrophages depending on the extent of the inflammatory reaction. Conclusions: The presence of many macrophages in the atherosclerotic plaques indicates a chronic inflammatory reaction, accompanied by fibroblast proliferation and connective tissue changes that influence the stability of the plaques.