动脉粥样硬化斑块中TNF-α的表达与斑块稳定性的关系

摘要

Objective To study the relationship between expression of tumor necrosis factor-α (TNF-α) and plaque stability in atherosclerotic plaques. Methods The patients with coronary atherosclerosis (n=80) undergone surgery were chosen from the Second People’s Hospital of Zhengzhou City from Feb. 2014 to Mar. 2017, and samples of atherosclerotic plaques were collected from the patients. The samples of atherosclerotic plaques were observed under optical microscope after HE staining, and then divided into normal control group, stable plaque group and unstable plaque group. The expressions of macrophage cells (CD68), smooth muscle cells (α-actin) and TNF-α in atherosclerotic plaques were detected by using immunohistochemistry technique. Results There was no CD68 expression and positive rate of α-actin was 100.00% in normal control group. The positive rate of CD68 was 60.71% in stable plaque group and 96.77% in unstable plaque group, and positive rate of α-actin was 53.57% in stable plaque group and 9.68% in unstable plaque group (P<0.05). There was no TNF-α expression in normal control group, and positive rate of intima TNF-α was 64.28% in stable plaque group and 93.54% in unstable plaque group, and positive rate of tunica media TNF-α was 28.57% in stable plaque group and 61.29% in unstable plaque group (P<0.05). Conclusion The expression of TNF-α can promote the instability of atherosclerotic plaques, and it is one of important factors in the formation and development of atherosclerotic lesions.%目的 研究动脉粥样硬化斑块中肿瘤坏死因子α(TNF-α)的表达与斑块稳定性的关系.方法 选取2014年2月至2017年3月于郑州市第二人民医院行手术治疗的冠状动脉粥样硬化患者80例中获取患者冠脉粥样硬化斑块标本,经HE染色后在光学显微镜下观察,对冠脉粥样斑块面积、脂核面积和纤维帽厚度进行测量分析,将其分为正常对照组、稳定斑块组和不稳定斑块组.采用免疫组织化学检测技术检测冠脉斑块中巨噬细胞(CD68)、平滑肌细胞(α-actin)、TNF-α的表达情况.结果 正常对照组无CD68的表达,α-actin的阳性率为100%,稳定斑块组与不稳定定斑块组阳性率分别为(60.71%, 53.57%)和(96.77%,9.68%),每两组间比较差异均有统计学意义(P＜0.05);TNF-α在正常对照组中无表达,在稳定斑块组与不稳定斑块组的内膜与中膜阳性率分别为(64.28%,28.57%)和(93.54%, 61.29%),每两组间差异均有统计学意义(P＜0.05).结论 TNF-α的表达能促进动脉粥样硬化斑块的不稳定性,是动脉粥样硬化病变形成和发展的重要因素之一.