The expression of matrix metalloproteinases-9, transforming growth factor- β1 and transforming growth factor- β receptor I in human atherosclerotic plaque and their relationship with plaque stability

摘要

Background Transforming growth factor-beta (TGF-β) and matrix metalloproteinases-9 (MMP-9) have been implicated in the pathogenesis of human atherosclerosis but their relationship during lesion progression are poorly understood. The objective of this study was to investigate the expression of MMP-9, TGF-β1 and TGF-β receptor I (TβR-I ) in human atherosclerotic plaque and their relationship and plaque stability. Methods Specimens of human coronary artery atherosclerotic plaques were obtained from 41 patients undergoing coronary endarterectomy, and were paraffin embedded, sectioned at 4 μm intervals then stained with haematoxylin and eosin. They were divided into stable (with no or only little lipid core) and unstable plaque groups (with lipid core size > 40%): the immunohistochemical staining were performed for MMP-9, TGF-β1 and TβR- I. Results The expression of MMP-9 in the unstable plaques was much higher than in the stable ones, but the expression of TGF-β1 was higher in the stable plaques. There was no similar significant difference for TβR- I. Correlation analysis showed that there was a negative correlation between the expression of MMP-9 and TGF-β1 (r= -0.332, P=0.034 for average areal density; r= -0.373, P = 0.016 for average optical density). Conclusions There were close relationships between MMP-9, TGF-β1 and plaque stability. Enhanced production of MMP-9 may participate in the formation of unstable plaque, while TGF-β1 maybe an important stabilizing factor in preventing transition into an unstable plaque phenotype.