Consequences of Soluble ICAM-1 N-Glycan Alterations on Receptor Binding and Signaling Kinetics in Mouse Astrocytes

摘要

Soluble intercellular adhesion molecule-1 (sICAM-1) is elevated in the cerebrospinal fluid of patients with severebrain trauma and mouse sICAM-1 induces the production of macrophage inflammatory protein-2 (MIP-2) in mouseastrocytes. The production of MIP-2 is greatly enhanced when sICAM-1 contains sialylated complex-type N-glycans(sICAM-1-CT) as produced by Chinese hamster ovary (CHO) cells. By contrast, sICAM-1 from the Lec1 mutant of CHOcells (sICAM-1-HM), containing only high mannose-type N-glycans, is relatively inactive. Here we show that the Nglycansof sICAM-1-CT are mostly α2,3-sialylated bi-, tri-, and tetraantennary complex-type structures with varyingamounts of core fucosylation. Unexpectedly, sICAM-1-CT and sICAM-1-HM bound equivalently to mouse astrocytes.Enhanced MIP-2 induction by sICAM-1-CT was associated with a more rapid, higher level, and prolonged MIP-2 responseas well as sICAM-1-CT accumulation at the plasma membranes of mouse astrocytes. These results show that glycosylationof sICAM-1 contributes to its signaling properties at the astrocyte cell surface, and suggest that altered glycosylationwhich might arise as a result of inflammation could regulate the bioactivity of sICAM-1.