MULTIPLE LOW LEVEL LASER IRRADIATION EFFECTS ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES AND PLATELETS REVEALED BY FLUORIMETRIC TECHNIQUES

摘要

The aim of the present study was to supply new data concerning the cellular effects of low power long wavelength laser irradiation. Steady state fluorescence intensity measurements with the use of appropriate fluorophores allowed disclosure of changes induced by the 680 nm red and the 830 nm infrared laser radiation in the polar headgroup region lipid order parameter of plasma membranes as well as in the intracellular calcium levels of human platelets and peripheral blood lymphocytes. Comparison of the effects seen in freshly separated and in metabolically impaired cells revealed a sensitive modulation of laser irradiation effects by the actual metabolic state of the cells.