Functional and morphological alteration in deoxinivalenol (DON) induced liver and kidney injuries

摘要

The aim of the study was to assess the influence of known concentration of deoxinivalenol (DON), naturally produced by fungi, on the blood component and histological structure in liver and kidney. Acting in small amounts they can be toxic to humans and animals. Forty male rats of the Sprague-Dowley breed were divided in 4 groups who received through feed doses of 3 mg DON/kg BW (B, n=10), 5 mg DON/kg BW (C, n=10), 9 mg DON/kg BW (D, n=10) and none DON (A-control group, n=10). The main components of blood as a alanine aminotransferase (ALT), glucose (GLU), serum creatinine (C), serum urea (U) and blood urea nitrogen (BUN) were significantly higher (P<0.05) and serum albumin (ALB) respectively total serum protein (TP) were significantly lower (P<0.05) for in experimental groups compared to control group. Complexity of pathological changes in liver and kidney increased with increasing dose DON administered in feed. In the liver tissues were highlighted with the following pathological changes: hyperemia and swelling of hepatic sinusoids, hepatocyte large globular with rich intense eosinophilic cytoplasm, enlarged nuclei with evident nucleolus and hyperchromasia variable light space portal fibrosis with rare inflammatory cells and swelling of blood vessels and accumulation of apoptotic bodies. In renal tissue the pathological changes were represented by degenerative lesions of the renal tubular epithelium and glomerulus level.