Epidemiología de la Proteína de Superficie Neumocócica a (PspA), en Streptococcus pneumoniae Causantes de Infecciones Invasivas en un Hospital Pediátrico de la Argentina

摘要

It has been emphasized Pneumococcal Surface Protein A (PspA) as a main component for developing apneumococcal vaccine with the potential to offer a broad protection unrelated to serotype. The aim of this work wasto determine the frequency and distribution of PspA families 1 and 2 of Streptococcus pneumoniae causing invasivepediatric infections. We worked with 91 isolates from the period 2005-2012. Antibiotic sensitivity was studiedaccording to the Clinical and Laboratory Standards Institute, serotyping by Quellung technique, and the presenceof PspA variant gen was determined by polymerase chain reaction. From the studied isolates, 48 (52.7%) wereidentified as family 1, 28 (30.8%) as family 2 and in 6 (6.6%) none of the desired amplicon was detected. Expressionof both families was found in 9 isolates (10%). Family 2 frequencies were higher than expected among children 0.05). Sixteen different serotypes were detected; serotype 1 was relatedto family 1 and serotype 7F to family 2. Serotype 14 was associated with both families. No difference betweenthe distribution of families and the results of minimum inhibitory concentration of penicillin and cefotaxime wasdetected. Our findings showed that the development of a PspA antigens based vaccine, containing both families1 and 2 would be effective in the region.