Effect of β-mercaptoethanol on in vitro maturation on oocyte of Murrah buffalo

摘要

This study was conducted to determine the effect of supplementing maturation medium with β-mercaptoethanol on in vitro maturation on oocytes of Murrah buffalo. Buffalo cumulus-oocyte complexes (COCs) from ovaries were matured in vitro in Hepes-TCM 199 supplemented with 0.2 mM sodium pyruvate, 1 μg/ml 17-β- estradiol, 10% fetal calf serum (FCS), 0.5 μg/ml bFSH and 0 (control) and 0.1 or 0.5 mM/ml of β-mercaptoethanol for 24 h. When COCs matured in TCM 199 media with 500 μM/ml β-mercaptoethanol , the rate of maturation increased significantly as compared to the control group (53.33 vs. 33.33%, respectively). Also, the percentage of degenerated oocytes in the treatment groups was lower than that in the control group (P<0.05).??