首页> 外文期刊>Research Journal of Applied Sciences: RJAS >Influence of Solid Loading Concentrations, Inoculums Size and Nitrogen Sources on Ethanol Production from Empty Fruit Bunches (EFB) Hydrolysate in Separate Hydrolysis and Fermentation (SHF)
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Influence of Solid Loading Concentrations, Inoculums Size and Nitrogen Sources on Ethanol Production from Empty Fruit Bunches (EFB) Hydrolysate in Separate Hydrolysis and Fermentation (SHF)

机译:固体负载浓度,接种量和氮源对单独水解和发酵(SHF)中空果束(EFB)水解产物生产乙醇的影响

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Malaysia is one of the largest palm oil producers in the world and the industry has contributed the significant income to the country for many years. With the rapid growth of palm oil production in Malaysia, the amount of biomass residues generated also has shown a corresponding increase. Mass generation of palm biomass particularly Empty Fruit Bunches (EFB) has been identified as one of the main environmental issue generated from palm oil industry. Conversion of EFB into value added products such as bioethanol has been identified as an alternative way to reduce accumulation of EFB in the plantation. In this study, the effects of different EFB concentration, inoculums size and nitrogen sources on bioethanol production using pre-treated EFB were investigated. The pulverized EFB was initially pre-treated with 1% (w/v) sodium hydroxide (NaOH) followed by acid hydrolysis using 0.7% (v/v) sulfuric acid (H2SO4) and enzymatic saccharification prior to fermentation via Separate Hydrolysis and Fermentation (SHF) mode with Saccharomyces cerevisiae were carried out. The enzymatic hydrolysis process of EFB indicated that the highest glucose concentration was obtained by using 15% (w/v) EFB with 26.35 g L-1 glucose and 4.6 g L-1 xylose, respectively. The fermentation of hydrolysate obtained from saccharified EFB at different solid loading indicated that the highest ethanol production was achieved at 10% (w/v) EFB with 10.45 g L-1 ethanol corresponding to ethanol yield (Yp/s) of 0.53 g g-1 glucose. The most suitable inoculums size for ethanol production from saccharified EFB hydrolysate was 10% (v/v) active S. cerevisiae and additional of peptone in the EFB hydrolysate was not significantly affected (p<0.5) the ethanol production. However, additional of peptone in the saccharified EFB hydrolysate was able to reduce period of fermentation from 72-48 h of incubation.
机译:马来西亚是世界上最大的棕榈油生产国之一,多年来该行业为该国贡献了可观的收入。随着马来西亚棕榈油产量的快速增长,生物质残留物的产生量也显示出相应的增加。棕榈生物质的大量产生,特别是空果束(EFB)已被确定为棕榈油工业产生的主要环境问题之一。已经确认将EFB转化为增值产品(例如生物乙醇)是减少人工林中EFB积累的替代方法。在这项研究中,研究了不同的EFB浓度,接种量和氮源对使用预处理的EFB的生物乙醇生产的影响。粉碎的EFB最初先用1%(w / v)的氢氧化钠(NaOH)进行预处理,然后使用0.7%(v / v)的硫酸(H2SO4)进行酸水解和酶促糖化,然后通过单独的水解和发酵进行发酵(用酿酒酵母(Saccharomyces cerevisiae)进行SHF)模式。 EFB的酶水解过程表明,通过分别使用15%(w / v)EFB和26.35 g L-1葡萄糖和4.6 g L-1木糖,可以获得最高的葡萄糖浓度。由糖化EFB在不同固体负载下获得的水解产物的发酵表明,在10%(w / v)EFB下,用10.45 g L-1乙醇可获得最高的乙醇产量,对应于0.53 g g-的乙醇收率(Yp / s)。 1葡萄糖。从糖化的EFB水解产物生产乙醇的最合适接种量为10%(v / v)活性酿酒酵母,EFB水解产物中额外的蛋白ept不会显着影响乙醇生产(p <0.5)。但是,糖化的EFB水解产物中添加的蛋白ept能够减少72-48小时的发酵时间。

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