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首页> 外文期刊>Lipids in Health Disease >Effect of 50?Hz electric field in diacylglycerol acyltransferase mRNA expression level and plasma concentration of triacylglycerol, free fatty acid, phospholipid and total cholesterol
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Effect of 50?Hz electric field in diacylglycerol acyltransferase mRNA expression level and plasma concentration of triacylglycerol, free fatty acid, phospholipid and total cholesterol

机译:50?Hz电场对二酰基甘油酰基转移酶mRNA表达水平和血浆三酰基甘油,游离脂肪酸,磷脂和总胆固醇浓度的影响

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Background The effects of exposure to a 50?Hz electric field (EF) on plasma level of triacylglycerol, free fatty acids, total cholesterol and phospholipid and mRNA expression level of diacylglycerol acyltransferase (DGAT) 1 and 2 in liver and intestines from C57BL/6?J mice were studied. Methods The test was based on comparison between mice post treated with 50?Hz EF of 45?kV/m intensity for 30?min per day for 11?days or without EF. DGATs mRNA expression was analyzed by real-time quantitative polymerase chain reaction. Results There was no difference in the gene expression level of DGAT1 in liver and intestines. The DGAT2 gene expression level in liver derived from mice treated with EF was significantly lower than those in the control (P?
机译:背景暴露于50?Hz电场(EF)对C57BL / 6肝脏和肠中血浆三酰甘油,游离脂肪酸,总胆固醇和磷脂水平以及二酰甘油酰基转移酶(DGAT)1和2 mRNA表达水平的影响研究了JJ小鼠。方法该试验基于在以50?Hz EF,45?kV / m强度每天处理30?min,连续11?天或没有EF的小鼠之间进行比较。通过实时定量聚合酶链反应分析了DGATs mRNA的表达。结果肝,肠中DGAT1基因表达水平无差异。用EF处理的小鼠肝脏中的DGAT2基因表达水平显着低于对照组(P <0.001)。暴露于EF组的血浆总胆固醇(P 0.01)和磷脂(P 0.05)均低于对照组,但三酰甘油或游离脂肪酸水平无差异。结论暴露于50?Hz EF会降低血浆中总胆固醇和磷脂的水平,并下调肝脏DGAT2 mRNA的表达。 EF对脂质代谢的作用机理尚不清楚,但可能涉及DGAT2活性的改变。

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