Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts

摘要

Background Isoprene, a volatile C5 hydrocarbon, is an important platform chemical used in the manufacturing of synthetic rubber for tires and various other applications, such as elastomers and adhesives. Results In this study, Escherichia coli MG1655 harboring Populus trichocarpa isoprene synthase (Pt ispS ) and the exogenous mevalonate (MVA) pathway produced 80?mg/L isoprene. Codon optimization and optimal expression of the ispS gene via adjustment of the RBS strength and inducer concentration increased isoprene production to 199 and 337?mg/L, respectively. To augment expression of MVA pathway genes, the MVA pathway was cloned on a high-copy plasmid (pBR322 origin) with a strong promoter (P_trc), which resulted in an additional increase in isoprene production up to 956?mg/L. To reduce the formation of byproducts derived from acetyl-CoA (an initial substrate of the MVA pathway), nine relevant genes were deleted to generate the E. coli AceCo strain ( E. coli MG1655 Δ ackA - pta , poxB , ldhA , dld , adhE , pps , and atoDA ). The AceCo strain harboring the ispS gene and MVA pathway showed enhanced isoprene production of 1832?mg/L in flask culture with reduced accumulation of byproducts. Conclusions We achieved a 23-fold increase in isoprene production by codon optimization of Pt ispS , augmentation of the MVA pathway, and deletion of genes involved in byproduct formation.