PRODUCTION OF LACCASE IN OLIVE OIL MILL WASTEWATER AND VINASSE BY IMMOBILIZED TRAMETES VERSICOLOR AND TRAMETES TROGII WITH DIFFERENT SUPPORTS

摘要

Laccase production ability of immobilized forms of Trametes trogii and Trametes versicolor in vinasse and olive oil mill wastewater (OOMW) was investigated. Immobilized fungi could be used repeatedly and successfully produced laccase enzyme under repeated-batch mode. While the highest laccase activity of T. trogii immobilized into alginate beads was 2.53 U/mL in vinasse after eleven cycles, the highest activity as 3.33 U/mL was obtained in OOMW after two cycles. The highest activities for T. trogii immobilized on pine cone particles (PCP) were 1.02 U/mL in vinasse and 3.02 U/mL in OOMW after five cycles and six cycles, respectively. On the other hand, the highest activity by T. versicolor immobilized into alginate beads was 1.28 U/mL in vinasse after fifteen cycles and 3.89 U/mL after two cycles in OOMW. For the fungus immobilized on PCP, the highest activities were 2.41 U/ml after two cycles and 3.29 U/mL after three cycles in vinasse and OOMW, respectively. Laccase production potential of the immobilized fungi in OOMW was higher than the forms in vinasse. The highest laccase activities obtained with the immobilized fungi were generally higher than the activities obtained by free cells. Immobilized fungi also reduced the COD of these wastewaters.