THE EFFECTS OF NARINGIN AND OZONE IN HEPATIC ISCHEMIA REPERFUSION INJURY: AN EXPERIMENTAL STUDY

摘要

Background and Aim. Hepatic ischemia reperfusion (I/R) injury is located between complications widely seen in clinical. Aim of this study was to investigate the protective roles of naringin and ozone effects and synergistic effects induced I/R injury on the liver in rats.Methods. Thirty five Adult male Sprague-Dawley rats were divided into five groups (n=7 in each group): sham-operated, I/R, I/R with 80 mg/kg naringin, I/R with 0.5 mg/kg ozone, and I/R with 80 mg/kg naringin+0.5 mg/kg ozone. Before hepatic I/R was induced, ozone and naringin was injected in-traperitoneally at doses of 80 mg/kg and 0.5 mg/kg. After 70-min ischemia and a 120-min reperfusion period, later at the end of experiment, liver tissues were excised. The levels of malondialdehyde (MDA) and activities of superoxide dismutase (SOD), glutathione reductase (GR), catalase (CAT) were measured in hepatic tissue.Results. SOD, GR, and CAT activities decreased and MDA level, as a biomarker of the lipid peroxidation, increased in I/R group compared to Sham-operated group. In addition, SOD, GR, and CAT activities increased by the naringin, ozone, and naringin+ozone treatment.Conclusions. The results of this study suggest that naringin, ozone, and naringin+ozone. may be strongly protective against hepatic I/R injury. This effect can be achieved by antioxidant and antiapop-totic activities.