Lipoteichoic Acid of Probiotic Lactobacillus plantarum Attenuates Poly I:C-Induced IL-8 Production in Porcine Intestinal Epithelial Cells

摘要

FIGURE 1. TLR3 ligand poly I:C induces IL-8 production in IPEC-J2 cells. IPEC-J2 cells (5 × 105 cells/ml) were plated in 96-well plates and treated with 1 or 10 μg/ml poly I:C (A) for 24 h in serum-free medium. Then, the supernatants were collected and IL-8 secretion was determined by ELISA. IPEC-J2 cells (5 × 105 cells/ml) were plated in 12-well plates and treated with 0.1, 1, or 10 μg/ml poly I:C for 3 h (B), or 1 μg/ml poly I:C for 0, 1, 3, 6, or 12 h in serum-free medium (C). After treatment, total RNA was extracted and IL-8 mRNA expression was determined by real-time PCR. Data are mean ± standard deviation of triplicate samples. (D) IPEC-J2 cells (5 × 105 cells/ml) were plated in 96-well culture plates and treated with 1 μg/ml poly I:C for 1, 3, 6, 12 and 24 h, and IL-8 secretion was determined by ELISA. Data are expressed as mean value ± standard deviation of triplicate samples. Asterisk (?) indicates significant difference compared with the non-treatment control group (P < 0.05).