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Evaluation of the impact of different drying methods on the phenolic compounds, antioxidant activity, and in vitro digestion of green coffee beans

机译:评价不同干燥方法对生咖啡豆中酚类化合物,抗氧化剂活性和体外消化的影响

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We investigated the effects of different drying methods (room temperature drying, heat pump drying, freeze drying, microwave vacuum drying [MVD], and combined microwave power vacuum drying) on the active ingredients of green coffee beans. We specifically focused on eleven phenolic acids, total phenolic content (TPC), total flavonoid content, antioxidant activity, polyphenol oxidase (PPO), the inhibition of linoleic acid peroxidation (LPO), and enzyme activity, and the bio‐accessibility in vitro and bioavailability of phenolics and antioxidant activities were also evaluated. MVD‐treated beans had the lowest PPO activity and the highest contents of 5‐caffeoylquinic acid (1.39?g/100?g?DW), 3‐feruloylquinic acid (0.25?g/100?g?DW), 4‐feruloylquinic acid (0.25?g/100?g?DW), 5‐feruloylquinic acid (1.52?g/100?g?DW), and TPC (5.98?g GAE/100?g DW), and the highest antioxidant activity. MVD was the least time‐consuming (0.63?hr/kg) and energy‐consuming (1.88?kwh/kg) method. Moreover, the strongest inhibition of LPO and α‐glucosidase was observed in MVD‐treated beans. Thus, MVD is suggested to be the most suitable drying technique for the preservation of phenolic compounds and bioactivities of green coffee beans.
机译:我们研究了不同干燥方法(室温干燥,热泵干燥,冷冻干燥,微波真空干燥[MVD]和组合微波功率真空干燥)对生咖啡豆活性成分的影响。我们专门研究了11种酚酸,总酚含量(TPC),总黄酮含量,抗氧化剂活性,多酚氧化酶(PPO),亚油酸过氧化(LPO)和酶活性的抑制以及体外和体外的生物利用度还评估了酚类的生物利用度和抗氧化活性。 MVD处理过的豆具有最低的PPO活性和最高的5-咖啡酰奎尼酸(1.39?g / 100?g?DW),3-阿魏酰奎尼酸(0.25?g / 100?g?DW),4-阿魏酰奎尼酸(0.25?g / 100?g?DW),5-阿魏酰基奎尼酸(1.52?g / 100?g?DW)和TPC(5.98?g GAE / 100?g DW),抗氧化活性最高。 MVD是最省时(0.63?hr / kg)和最耗能(1.88?kwh / kg)的方法。此外,在MVD处理过的豆子中,对LPO和α-葡萄糖苷酶的抑制作用最强。因此,MVD被认为是最适合保存酚类化合物和生咖啡豆生物活性的干燥技术。

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