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Aflatoxin and Genetically Modified Organisms Analysis in Turkish Corn

机译:土耳其玉米中的黄曲霉毒素和转基因生物分析

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Genetically Modified Organisms (GMOs) are plants, animals or microorganisms that have been altered using genetic insertion or deletion via technology (also called genetic engineering or GE). Corn is one of the main inputs of both food and feed production. Also it is a major commodity of which many transgenic varieties are commercially available. In this study, the aflatoxin and GMO analyses of corn were made in two different years (2013 and 2014). Corn samples were collected between August and October in 2013 and 2014 around Turkey from non-neighboring fields representing each 10 thousand tons production and blending. After the analyses, which were performed by using LC-MS/MS technique, 38 of 538 corn samples (7.06%) were found to contain aflatoxin in 2013. 17 of these were collected from the first harvest, 21 were from the second harvest period. Aflatoxin limits were exceeded in samples from only 2 regions in 2014. This study also reports results of GMO screening tests with 0.05% detection limit in two marketing years. 35D promoter, NOS terminator and FMV promoter are sought with the tests. None of the samples shows positive results, that is, samples do not contain GMO.
机译:转基因生物(GMO)是指通过技术(也称为基因工程或GE)使用基因插入或缺失进行了改变的植物,动物或微生物。玉米是粮食和饲料生产的主要投入之一。它也是其中许多转基因品种可商购的主要商品。在这项研究中,玉米的黄曲霉毒素和GMO分析是在两个不同的年份(2013年和2014年)进行的。 2013年8月至10月之间以及2013年至2014年10月间,在土耳其附近的非相邻田地采集了玉米样品,代表每万吨谷物的生产和混合。在使用LC-MS / MS技术进行分析之后,2013年发现538个玉米样品中有38个(7.06%)含有黄曲霉毒素。其中17个来自第一次收获,21个来自第二次收获期。 2014年只有2个地区的样品中黄曲霉毒素含量超标。该研究还报告了在两个销售年度中GMO筛查结果的检出限为0.05%。测试中寻求35D启动子,NOS终止子和FMV启动子。所有样品均未显示阳性结果,即样品不含GMO。

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