Genome comparison of Listeria monocytogenes serotype 4a strain {HCC23} with selected lineage I and lineage {II} L. monocytogenes strains and other Listeria strains

摘要

Abstract More than 98% of reported human listeriosis cases are caused by specific serotypes within genetic lineages I and II. The genome sequence of Listeria monocytogenes lineage {III} strain {HCC23} (serotype 4a) enables whole genomic comparisons across all three L. monocytogenes lineages. Protein cluster analysis indicated that strain {HCC23} has the most unique protein pairs with nonpathogenic species Listeria innocua. Orthology analysis of the genome sequences of representative strains from the three L. monocytogenes genetic lineages and L. innocua (CLIP11262) identified 319 proteins unique to nonpathogenic strains {HCC23} and {CLIP11262} and 58 proteins unique to pathogenic strains {F2365} and EGD-e. {BLAST} comparison of these proteins with all the sequenced L. monocytogenes and L. innocua revealed 126 proteins unique to serotype 4a and/or L. innocua; 14 proteins were only found in pathogenic serotypes. Some of the 58 proteins unique to pathogenic strains {F2365} and EGD-e were previously published and are already known to contribute to listerial virulence.