Complete Genome Sequence of Cell Culture-Attenuated Guinea Pig Cytomegalovirus Cloned as an Infectious Bacterial Artificial Chromosome

Dongmei Yang; Zohaib Alam; Xiaohong Cui; Michael Chen; Carly J. Sherrod; Michael A. McVoy

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Complete Genome Sequence of Cell Culture-Attenuated Guinea Pig Cytomegalovirus Cloned as an Infectious Bacterial Artificial Chromosome

机译：完整的基因组序列的克隆的感染性细菌人工染色体细胞培养的豚鼠巨细胞病毒的克隆

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The complete genome sequence of attenuated guinea pig cytomegalovirus cloned as bacterial artificial chromosome N13R10 was determined. Comparison to pathogenic salivary gland-derived virus revealed 13 differences, 1 of which disrupted overlapping open reading frames encoding GP129 and GP130. Attenuation of N13R10 may arise from an inability to express GP129 and/or GP130.

机译：确定了克隆为细菌人工染色体N13R10的减毒豚鼠巨细胞病毒的完整基因组序列。与致病性唾液腺衍生病毒的比较显示13种差异，其中1种破坏了编码GP129和GP130的重叠阅读框。 N13R10的减弱可能是由于无法表达GP129和/或GP130而引起的。

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《Genome Announcements》 |2014年第5期|共2页
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Dongmei Yang; Zohaib Alam; Xiaohong Cui; Michael Chen; Carly J. Sherrod; Michael A. McVoy;
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中图分类基因工程（遗传工程）;
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1. Complete Genome Sequence of Cell Culture-Attenuated Guinea Pig Cytomegalovirus Cloned as an Infectious Bacterial Artificial Chromosome [J] . Dongmei Yang, Zohaib Alam, Xiaohong Cui, Genome Announcements . 2014,第5期

机译：完整的基因组序列的克隆的感染性细菌人工染色体细胞培养的豚鼠巨细胞病毒的克隆
2. Cloning the complete guinea pig cytomegalovirus genome as an infectious bacterial artificial chromosome with excisable origin of replication. [J] . Cui X, McGregor A, Schleiss MR, Journal of Virological Methods . 2008,第2期

机译：将完整的豚鼠巨细胞病毒基因组克隆为具有可复制复制起点的传染性细菌人工染色体。
3. Molecular cloning of the guinea pig cytomegalovirus (GPCMV) genome as an infectious bacterial artificial chromosome (BAC) in Escherichia coli. [J] . McGregor A, Schleiss MR Molecular genetics and metabolism . 2001,第1期

机译：几内亚猪巨细胞病毒（GPCMV）基因组的分子克隆，作为大肠杆菌中的感染细菌人工染色体（BAC）。
4. High throughput design of Bacterial Artificial Chromosomes (BACs) for cell type specific gene expression in transgenic mice [C] . David Balya, Botond Roska IEEE Biomedical Circuits and Systems Conference . 2006

机译：转基因小鼠细胞类型特异性基因表达的细菌人工染色体（BACS）的高通量设计
5. Characterization of a bacterial artificial chromosome (BAC)-based infectious clone of a low passage Marek's disease virus (MDV) vaccine strain, CVI988. [D] . McDowell, Erin. 2009

机译：低传代马立克氏病病毒（MDV）疫苗株CVI988基于细菌人工染色体（BAC）的传染性克隆的特征。
6. Complete Genome Sequence of Cell Culture-Attenuated Guinea Pig Cytomegalovirus Cloned as an Infectious Bacterial Artificial Chromosome [O] . Dongmei Yang, Zohaib Alam, Xiaohong Cui, 2014

机译：完整的基因组序列的细胞培养的豚鼠巨细胞病毒克隆为感染性细菌人工染色体。
7. Complete Genome Sequence of Cell Culture-Attenuated Guinea Pig Cytomegalovirus Cloned as an Infectious Bacterial Artificial Chromosome [O] . Dongmei Yang, Zohaib Alam, Xiaohong Cui, 2014

机译：细胞培养的完整基因组序列 - 减毒的豚鼠缩细胞病毒作为传染性细菌人工染色体克隆

Complete Genome Sequence of Cell Culture-Attenuated Guinea Pig Cytomegalovirus Cloned as an Infectious Bacterial Artificial Chromosome

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