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首页> 外文期刊>European review for medical and pharmacological sciences. >Centromere protein U promotes cell proliferation, migration and invasion involving Wnt/β-catenin signaling pathway in non-small cell lung cancer
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Centromere protein U promotes cell proliferation, migration and invasion involving Wnt/β-catenin signaling pathway in non-small cell lung cancer

机译:着丝粒蛋白U促进非小细胞肺癌中涉及Wnt /β-catenin信号通路的细胞增殖,迁移和侵袭

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OBJECTIVE: The purpose of this study was to examine centromere protein U (CENPU) expression in non-small cell lung cancer (NSCLC) and identify the clinical values of CENPU, as well as investigate the potential molecular mechanisms in NSCLC. PATIENTS AND METHODS: The expression levels and clinical significance of CENPU were systematically evaluated in human protein atlas datasets and TCGA datasets. CENPU protein expression was studied by Western blotting. CENPU mRNA expression was studied by Real Time-Polymerase Chain Reaction (RT-PCR). Proliferation, migration, and invasion capacities of CENPU cells were assessed after silencing CENPU. Apoptosis was determined using flow cytometry. Western blotting was performed to assess the protein expression levels. RESULTS: We found that the expression of CENPU at mRNA and protein levels was significantly up-regulated in both NSCLC tissues and cell lines. Overexpression of CENPU was significantly associated with poor prognosis of NSCLC patients. Knockdown of CENPU significantly suppressed proliferation, migration, and invasion, and caused apoptosis of NSCLC cells in vitro. In addition, knockdown of CENPU suppressed epithelial-mesenchymal transition (EMT). Furthermore, our results revealed that the abnormal expression of CENPU could influence the Wnt/β-catenin signaling pathway. CONCLUSIONS: CENPU was highly expressed in NSCLC tissues and its knockdown of CENPU strongly suppressed NSCLC cell proliferation and metastasis through modulating Wnt/β-catenin signaling. Targeting CENPU could be a promising therapeutic strategy for patients with CENPU.
机译:目的:研究非小细胞肺癌(NSCLC)中着丝粒蛋白U(CENPU)的表达,鉴定其临床价值,并探讨其潜在的分子机制。患者和方法:在人蛋白质图谱数据集和TCGA数据集中系统地评估了CENPU的表达水平和临床意义。通过蛋白质印迹研究了CENPU蛋白的表达。通过实时聚合酶链反应(RT-PCR)研究了CENPU mRNA的表达。沉默CENPU后评估CENPU细胞的增殖,迁移和侵袭能力。使用流式细胞仪测定细胞凋亡。进行蛋白质印迹以评估蛋白质表达水平。结果:我们发现NSCLC组织和细胞系中CENPU在mRNA和蛋白水平的表达均显着上调。 CENPU的过度表达与NSCLC患者预后差有关。抑制CENPU可以显着抑制增殖,迁移和侵袭,并在体外引起NSCLC细胞凋亡。另外,CENPU的敲低抑制了上皮-间质转化(EMT)。此外,我们的结果表明,CENPU的异常表达可能影响Wnt /β-catenin信号通路。结论:CENPU在NSCLC组织中高表达,其敲低CENPU通过调节Wnt /β-catenin信号转导强烈抑制NSCLC细胞的增殖和转移。针对CENPU的靶向治疗可能是CENPU患者的一种有前途的治疗策略。

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