Histological and histochemical changes induced by the amendment of chitosan and inoculation of Pseudomonas sp. against Tomato leaf curl virus (ToLCV) in tomato (Lycopersicon esculentum Mill.)

摘要

This study was conducted to evaluate the comparison between the histological and histochemical changes in leaves of tomato due to tomato leaf curl virus (ToLCV) with or without plant growth-promoting rhizobacteria (PGPR) and chitosan treatments. The combined treatment of chitosan and Pseudomonas sp. induced a significant (P=0.05) increase in the activities of polyphenoloxidase (PPO), peroxidase (POD), chitinase, phenylammonialyase (PAL) and enhanced the content of phenolic compounds in tomato leaves. Furthermore, the combined application reduced the disease severity (%) of ToLCV in tomato plants. Although, the histopathological and histochemical studies revealed more destruction in the diseased plants, these changes could be reduced to the level of the healthy plants due to combined treatment of chitosan and Pseudomonas sp.