Application of PCR-RFLP of gap gene method as a molecular typing tool for coagulase negative Staphylococci from bovine and human origin identified with VITEK 2

摘要

The aim of this study was to apply the Restriction Fragment Length Polymorphism (RFLP) of Glyceraldehyde-3-Phosphate Dehydrogenase encoding gene (gap) for testing its performance as a molecular typing tool in coagulase negative staphylococci (CNS) isolates from bovine mastitis (n = 59) and human clinical cases (n = 13) identified with VITEK 2. According to the phenotypic identification results, bovine mastitis isolates were?Staphylococcus?haemolyticus,Staphylococcus?simulans,?Staphylococcus?auricularis,?Staphylococcus?warneri,Staphylococcus?hominis,?Staphylococcus?capitis,?Staphylococcus?xylosus,Staphylococcus?epidermidis?and?Staphylococcus?cohnii. Although most of those isolates were generated PCR amplicons with?gap?gene specific primers, PCR amplification of?gap?gene failed in 29 from 72 isolates. The samples that did not produce amplicons were reamplified with Staphylococcal 16S rRNA gene specific primers. After PCR amplifications, amplicons were produced in 17 from 29 samples. Three different restriction endonucleases (AluI, MseI and RsaI) were used for PCR-RFLP analysis, among these AluI has been found the most discriminatory power for identification in species. The results of the RFLP of?gapgene provide a support for the misidentification problem associated with VITEK 2 system for?S.?simulans,?S.?auricularis?and?S.?capitis?species. Moreover, more frequent failure in?gap?gene amplification for bovine isolates which were phenotypically identified as?S.?simulans, S.?auricularis, S.?capitis,?S.?xylosus?andS.?cohnii?was not clear. In addition, the method verified the phenotypic identification for?S.?haemolyticus,?S.?warneri,?S.?hominis?and?S.?epidermidisisolates with different rates at 100, 33.3, 57.1, and 66.7%, respectively.