Evaluation of the correlation between Aflatoxin B1 production and Aspergillus contamination in rice bran from northern Iran

摘要

Aflatoxins are potent mycotoxins, which are produced by toxigenic?Aspergillus?species including?Aspergillus?flavus?and?Aspergillus?parasiticus. The goals of this study were to characterize?Aspergillus?fungus isolated from rice bran and to determine the correlation between Aflatoxin B1?(AFB1) production and?Aspergillus?contamination in the samples. Thirty rice bran samples were collected from different regions of Mazandaran province, northern Iran. Samples containing 15 specimens were conserved for one year in the storage and the other one was not subjected to storage. Mycological analysis of rice bran was performed and AFB1?was purified from the samples and quantified by high performance liquid chromatography (HPLC) method with fluorimetry detection. The results of this study showed that almost all collected samples contained AFB1. The averages of AFB1?in new and old rice bran samples were found to be 5.07 and 6.81 μg/kg, respectively. No significant difference was obtained between AFB1?value in new and old samples. In addition, the correlation between culture results and aflatoxin production were significantly observed only in old samples (P<0.05). The most frequent isolatedAspergillus?species were?Aspergillus?terreus,?A. flavus,?Aspergillus?niger,?A. parasiticusand?Aspergillus?fumigatus. In conclusion, these results confirmed that rice bran could be contaminated with AFB1?and toxigenic?Aspergillus?species and human beings are in danger of been affected by contaminated rice products.