In vitro regeneration in pepper ( Capsicum annuum L.) and characterization of plant-regenerants

摘要

The regeneration answer of hypocotyl and cotyledon explants of two pepper genotypes on MS0 medium content of macro- and micro- nutrients – ? MS0, 1 ? MS0, 1 ? MS0 1 ? MS0 and MS0 (control) with 3.0 mgL-1 BA, 0.3 mgL-1 IAA, 0.3 mgL-1 GA3 was studied. It has been found higher frequency of indirect regeneration in control culture medium while differentiation and elongation of obtained shoots has been established in culture medium variant with 1 ? MS0 + 0.2 mgL-1 AgNO3 and 0.3 mgL-1 GA3. In R0 generation the regenerants were characterized with reduced plant height, leaf size, fruit weight and seeds per fruit. Statistical analysis of fruits morphology and productivity per plant in the next R1 generation indicated variation between lines and compared to parents. Most lines were with shorter, but wider fruits with thicker pericarp and lower productivity per plant. For breeding purposes the complex of traits is of interest for following investigation deserve lines 3/13 and 2-1/13 from initial variety Yasen F1. Observed differences confirm the possibility for using of somaclonal variation as a method for improving and enriching the diversity in pepper.