Rapid Detection of Foot and Mouth Disease Virus from Tongue Epithelium of Cattle and Buffaloes in Suez Canal Area, Egypt from 2009-2011

摘要

Detection of FMDV in clinical samples using virus isolation, ELISA and RT-PCR has been done during 2009-2011 period. Forty four Tongue epithelial samples were collected; 23, 16 and 4 samples from Ismailia, Suez and Port Said governorates respectively. Virus isolation of FMDV from Tongue epithelium samples in BHK 21 cell culture revealed that there were 42 positive samples out of 44 with 95.45% infectious rate. The results of ELISA of FMDV types O and A from tongue epithelium were 79.54 and 15.91%, respectively. Using ELISA, the percentage of FMDV type O in Ismailia, Suez and Port Said governorates were 82.61, 76.47 and 75%, respectively while FMDV type A in Ismailia, Suez and Port Said governorates were 13.04, 17.65 and 25%, respectively. The results of RT-PCR of FMDV types O and A FMDV were 81.81 and 15.91%, respectively. RT-PCR results of FMDV type O in Ismailia, Suez and Port Said governorates were 86.96, 76.47 and 75%, respectively whereas FMDV type A in Ismailia, Suez and Port Said governorates were 13.04, 17.65 and 25%, respectively. The results concluded that both FMDV types O and A are circulating in all localities of Suez Canal area, Ismailia (Abu-Sweir, El-Kasasin, El-Tall El-Kebir and Abu-Atwa), Suez (Suez, Kasfarite and Geniva) and Port Said (Port Said). Also, the results showed that RT-PCR is valuable sensitive test for detection of FMDV in clinical samples.