NON-TRANSMISSION OF FOOT AND MOUTH DISEASE VIRUS BY EMBRYOS FROM FOOT AND MOUTH DISEASE SEROPOSITIVE SHEEP AND CONVALESCENT GOATS

摘要

The research performed aims to search the potential risk of transmission of foot-and-mouth disease virus (FMDV) through embryos obtained from FMD seropositive donor sheep and FMD convalescent donor goats (experimentally inoculated) and transferred tofree recipient animals. Two experiments were performed in sheep. In experiment No. 1, seventy nine ewes and four rams were used and in experiment 2 , thirty three ewes and four rams. In both experiments, animals were selected from an endemic area from farms where an outbreak of FMD was reported. The ewes in both experiments were then subject to a 14 day heat synchronization. The donors were subjected to a superovulatory treatment and insemination. The embryos were collected by surgical technique. A group of goats consisting of 48 does and 3 bucks were confined in the maximum isolation units and infected with FMD virus. A strain of 01 was inoculated by intradermalingual route. The animals developed clinical signs and became positive to viral infection associated antigen (VIAA) after normal course of the disease and the virus was isolated from oesophageal-pharyngeal fluids (OPP). Heat synchronization, superovalutory treatment and insemination were performed and the embryos were collected by surgical technique.